ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of Ruiqi tablet on mitochondrion activities in the cerebral cortex neurons of fetal mice.</p><p><b>METHOD</b>The cerebral cortex of fetal Wistar rats after 16- 17 gestation days were collected and randomized into Ruiqi tablet group and blank control group after 4-5 days' culture. Laser scanning confocal microscope was adopted to determine the changes in the mitochondrion activities of the primary cultured cerebral cortex neurons of fetal rats after addition of Ruiqi tablet solution.</p><p><b>RESULT</b>Ruiqi tablet can increase the mitochondrion activities of the cerebral cortex neurons. No significant change in the mitochondrion activities of the cerebral cortex neurons was found in the blank control group.</p><p><b>CONCLUSION</b>Ruiqi tablet can increase the mitochondrion activities of the neurons and it has certain application prospects in treatment of some neurodegenerative diseases such as Alzheimer's disease, vascular dementia, Parkinson's disease and so on.</p>
Subject(s)
Animals , Female , Humans , Rats , Cerebral Cortex , Cell Biology , Drugs, Chinese Herbal , Pharmacology , Fetus , Chemistry , Microscopy, Confocal , Mitochondria , Physiology , Models, Animal , Neurons , Physiology , Random Allocation , Rats, Wistar , TabletsABSTRACT
Objective To study the effective sterilization method of laparoscopic instruments. Methods Using 2 different methods to sterilize the same laparoscopic instruments,and then compared the effects of sterilization. Results The bactericial rate of the 2 methods were both 100%. Conclusion The Huiri sterilizing method only need 40 minutes,which fits the requirement of one by one operation.
ABSTRACT
To explore the effect of Ruiqi pill on neuronal calcium level of cerebral cortex in brain of rat embryos.Methods: The cerebral cortex of rat embryos pregnanted for 16-17 days were dissected.The neural cells were divided into Ruiqi pill group and control group randomly after 4-5 days in vitro culture.Effect of Ruiqi pill on neuronal calcium level of primary culture of cortical neurons in rat embryos was measured by laser scanning confocal microscopy.Results:Ruiqi pill could reduce neuronal calcium concentration,no change was found in the control group.Conclusion: Ruiqi pill reduced neuronal calcium concentration,which might be one of the mechanisms of Ruiqi pill in improving memory function.